Anti-Human 5T4 Recombinant Antibody (SAA0571) (HC281207)

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货号	HC281207
品牌	abinScience
种属反应性	Human
应用	ELISA, FCM, SPR
同种型	IgG2a
克隆号	SAA0571
经测试应用	FCM
靶标	5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q13641
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Note	For research use only.

Bioactivity

Detects TPBG/5T4 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human TPBG antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # HC281207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human TPBG antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # HC281207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human TPBG antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TPBG monoclonal antibody (Catalog: HC281207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects Recombinant Human TPBG/5T4 Protein, C-His (Cat No.: HC281011) in indirect ELISA.

产品说明书

Anti-Human 5T4 Recombinant Antibody (SAA0571).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

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Anti-Human 5T4 Recombinant Antibody (SAA0571) (HC281207)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human 5T4 Recombinant Antibody (SAA0571) (HC281207)

概述

图片

参考文献

产品说明书

相关产品推荐

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