| 货号 | HB185307 |
|---|---|
| 品牌 | abinScience |
| 种属反应性 | Human |
| 应用 | FCM |
| 宿主 | Mouse |
| 同种型 | IgG2a, kappa |
| 克隆号 | SAA1404 |
| 克隆类型 | Monoclonal |
| 靶标 | Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3 |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| Accession号 | P20138 |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
SEC-HPLC detection for Anti-Human CD33 Antibody (ABS0480).
Flow-cytometry using anti-human CD33 antibody.CD33 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD33 antibody.RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 antibody monoclonal antibody (Catalog # HB185307 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD33 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD33 monoclonal antibody (Catalog: HB185307) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD33 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 monoclonal antibody (Catalog: HB185307, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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