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Anti-Human Integrin beta 1/CD29 Recombinant Antibody (TS2/16) (HY248207)

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  • 100ug
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  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HY248207
品牌abinScience
种属反应性Human
应用FCM
同种型IgG1, kappa
克隆号TS2/16
经测试应用FCM
靶标MSK12, VLA-4 subunit beta, CD29, Integrin beta-1, ITGB1, FNRB, MDF2, GPIIA, Glycoprotein IIa, Fibronectin receptor subunit beta
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P05556
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Integrin beta-1 (CD29/ITGB1) is a ~88 kDa protein. Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen.

1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415
2. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099
3. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536
4. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877
5. Bax, DV. et al. (2003) The Journal of biological chemistry 278, 34605-16. PMID: 12807887
6. Jovanovic, J. et al. (2007) The Journal of biological chemistry 282, 6743-51. PMID: 17158881
7. Schumacher, S. et al. (2021) Science advances 7. PMID: 33962943
8. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 29030430
9. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973
10. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
NoteFor research use only.
图片
  • Bioactivity

    Detects Human CD29/ITGB1 in indirect ELISAs.

  • Flow-cytometry

    Flow-cytometry using anti-human CD29 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow-cytometry

    Flow-cytometry using anti-human CD29 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD29 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD29 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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