Anti-Human CD5 Recombinant Antibody (H65), FITC (HY046117)

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货号	HY046117
品牌	abinScience
种属反应性	Human
应用	FCM, IF, WB
宿主	Mouse
同种型	IgG1, kappa
克隆号	H65
偶联物	FITC
克隆类型	Monoclonal
经测试应用	FCM
靶标	Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1
内毒素水平	Please contact with the lab for this information.
相关产品	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), FITC
Accession号	P06127
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Flow Cytometry

Flow-cytometry using FITC anti-human CD5 antibody. Jurkat cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-human CD5 antibody monoclonal antibody (Catalog # HY046117, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD5 Recombinant Antibody (H65), FITC.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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Anti-Human CD5 Recombinant Antibody (H65), FITC (HY046117)

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图片

参考文献

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重组蛋白

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实验相关试剂

Anti-Human CD5 Recombinant Antibody (H65), FITC (HY046117)

概述

图片

参考文献

产品说明书

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