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Anti-Human MIF Recombinant Antibody (SAA0115), FITC (HB816117)

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  • 50T
  • 100T
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概述
货号HB816117
品牌abinScience
种属反应性Human
应用FCM
宿主Human
同种型IgG1, kappa
克隆号SAA0115
偶联物FITC
克隆类型Monoclonal
靶标MIF, L-dopachrome isomerase, L-dopachrome tautomerase, GIF, Macrophage migration inhibitory factor, MMIF, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF
内毒素水平Please contact with the lab for this information.
相关产品 Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Accession号P14174
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Macrophage migration inhibitory factor (MIF/GIF) is a ~12 kDa protein. Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

1. Oddo, M. et al. (2005) Infection and immunity 73, 3783-6. PMID: 15908412
2. Emonts, M. et al. (2007) Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 44, 1321-8. PMID: 17443469
3. Fan, C. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10994-9. PMID: 23776208
4. Tan, TH. et al. (2001) The Biochemical journal 357, 373-83. PMID: 11439086
文献1. Stamps SL, et al. Mechanism of the phenylpyruvate tautomerase activity of macrophage migration inhibitory factor: properties of the P1G, P1A, Y95F, and N97A mutants. Biochemistry. 2000 Aug 15;39(32):9671-8.8 [HB816117]
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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