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Anti-Human CD9 Recombinant Antibody (SAA0003), FITC (HB984117)

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规格:
  • 50T
  • 100T
数量:
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概述
货号HB984117
品牌abinScience
种属反应性Human
应用ELISA, FCM, WB
宿主Human
同种型IgG1
克隆号SAA0003
偶联物FITC
克隆类型Monoclonal
经测试应用FCM
靶标Motility-related protein, Leukocyte antigen MIC3, TSPAN29, Cell growth-inhibiting gene 2 protein, CD9, MIC3, p24, Tspan-29, MRP-1, 5H9 antigen, Tetraspanin-29, CD9 antigen, RHD48801
内毒素水平Please contact with the lab for this information.
相关产品 Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Accession号P21926
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

CD9 antigen is a ~25 kDa protein. Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption.

1. Higginbottom, A. et al. (2003) Biochemical and biophysical research communications 311, 208-14. PMID: 14575715
2. Nakazawa, Y. et al. (2008) Blood 112, 1730-9. PMID: 18541721
3. Ikeyama, S. et al. (1993) The Journal of experimental medicine 177, 1231-7. PMID: 8478605
4. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
5. Masellis-Smith, A. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2768-77. PMID: 7511626
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow cytometry

    Flow-cytometry using FITC anti-human CD9 antibody. HCT-116 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD9 monoclonal antibody (Catalog HB984117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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