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Anti-Human CD163 Recombinant Antibody (AB101), FITC (HV831217)

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规格:
  • 50T
  • 100T
数量:
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概述
货号HV831217
品牌abinScience
种属反应性Human
应用FCM
宿主Mouse
同种型IgG2a, kappa
克隆号AB101
偶联物FITC
克隆类型Monoclonal
经测试应用FCM
靶标Scavenger receptor cysteine-rich type 1 protein M130, M130, Hemoglobin scavenger receptor, CD163, sCD163
内毒素水平Please contact with the lab for this information.
相关产品 Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), FITC
Accession号Q86VB7
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Scavenger receptor cysteine-rich type 1 protein M130 (CD163) is a ~125 kDa protein. Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow Cytometry

    Flow-cytometry using FITC anti-Human CD163 antibody. CACO-2 cells were stained with an irrelevant antibody (Green Histogram) or a FITC anti-Human CD163 antibody monoclonal antibody (Catalog # HV831217 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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