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Anti-Human CD350/FZD10 Recombinant Antibody (ONC-005), FITC (HV512117)

价格:
规格:
  • 50T
  • 100T
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HV512117
品牌abinScience
种属反应性Human
应用ELISA, FCM
宿主Human
同种型IgG1, kappa
克隆号ONC-005
偶联物FITC
克隆类型Monoclonal
靶标FZD10, Fz-10, hFz10, Frizzled-10, CD350, FzE7
内毒素水平Please contact with the lab for this information.
相关产品 Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Accession号Q9ULW2
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Frizzled-10 (CD350/FZD10) is a ~65 kDa protein. Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins.

NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow cytometry

    Flow-cytometry FITC using anti-human CD350 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD350 monoclonal antibody (Catalog HV512117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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