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Anti-Human E-Cadherin Recombinant Antibody (67A4), APC (HY460137)

价格:
规格:
  • 50T
  • 100T
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HY460137
品牌abinScience
种属反应性Human
应用FCM, IF
宿主Mouse
同种型IgG1, kappa
克隆号67A4
偶联物APC
克隆类型Monoclonal
经测试应用FCM
靶标CD324, Uvomorulin, Epithelial cadherin, E-cadherin, CDHE, CAM 120/80, UVO, CDH1, Cadherin-1
内毒素水平Please contact with the lab for this information.
相关产品 Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Accession号P12830
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Cadherin-1 (CD324/CDH1) is a ~97 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques.

1. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333
2. Shafraz, O. et al. (2018) eLife 7. PMID: 29999492
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow CytoMetry

    Flow-cytometry using APC anti-human CD324 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD324 monoclonal antibody (Catalog: HY460137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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