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Untouched Mouse Naïve Helper T Cell Isolation Kit (ICH3043CF)

价格: --
规格:
  • 100T
数量:
  • 概述

  • 图片

  • 参考文献

概述
货号ICH3043CF
品牌IchorBio
描述
Untouched Mouse Naïve Helper T Cell Isolation Kit (ICH3043CF) is a antibody targeting Naïve CD4+ T cell. Reactive with Mouse. Validated for Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research).
Highlights
  • ●Validated Applications — Tested in Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research).
种属反应性Mouse
应用Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research)
克隆类型Monoclonal
经测试应用Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research)
靶标Naïve CD4+ T cell
特异性Naïve CD4+ T cell
Accession号P06332
状态Liquid
运输Blue Ice
产品使用信息
应用方法 稀释比例
Ideal for isolating primary, untouched naïve CD4+ T cells for immunology research, functional T cell assays, and cell therapy applications requiring high purity and preserved native function.
稳定性和存储Store at 2–8°C; do not freeze
规格Size: 100T
背景

Naïve CD4+ T cells (CD4+CD62L+CD44-) are antigen-inexperienced helper T lymphocytes that have not yet encountered cognate antigen, representing a critical starting population for studies of T cell priming, differentiation, and adaptive immune responses. Their unactivated, quiescent state is highly sensitive to the isolation method used, making unmanipulated recovery essential for downstream functional and differentiation assays. The Ichorbio Untouched Mouse Naïve Helper T Cell Isolation Kit purifies naïve CD4+ T cells from mouse splenocytes or lymph node cells via column-free negative (untouched) selection, using a biotin-conjugated antibody cocktail and magnetic beads to label and sequester non-target and non-naïve cells, allowing untouched naïve CD4+ T cells to be recovered directly from the supernatant. This yields high-purity, untouched cells free of surface-bound antibodies or beads, with native phenotype and activation status preserved for sensitive downstream applications.

Cd4 is expressed on helper T lymphocytes and thymocyte subsets in mouse; naive CD4+ T cells are additionally CD62L-high/CD44-low, distinguishing them from memory/effector subsets.

NoteResearch Use Only (RUO)
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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