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Indirect Human B Cell Depletion Kit (ICH3056CB)

价格: --
规格:
  • 100T
数量:
  • 概述

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  • 参考文献

概述
货号ICH3056CB
品牌IchorBio
描述
Indirect Human B Cell Depletion Kit (ICH3056CB) is a antibody targeting CD19 (B cell). Reactive with Human. Validated for Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research).
Highlights
  • ●Validated Applications — Tested in Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research).
种属反应性Human
应用Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research)
克隆类型Monoclonal
经测试应用Cell isolation / separation (flow cytometry, cell culture, functional & cell-therapy research)
靶标CD19 (B cell)
特异性CD19 (B cell)
Accession号P15391
状态Liquid
运输Blue Ice
产品使用信息
应用方法 稀释比例
Ideal for immunology research, cell therapy development, and functional studies requiring B cell-depleted human cell populations with preserved viability and native immune function.
稳定性和存储Store at 2–8°C; do not freeze
规格Size: 100T
背景

B cells (CD19+) are the primary mediators of humoral immunity, responsible for antibody production, antigen presentation, and immune memory, and their selective depletion enables the enrichment of untouched non-B cell populations for studies where B cell contamination would confound downstream analysis. Removing this population without disturbing the target cells' physiological state is essential for applications such as T cell or NK cell functional assays and immune profiling. The Ichorbio Indirect Human CD19+ B Cell Depletion Kit depletes CD19+ B cells from PBMCs via a column-based indirect magnetic labeling strategy, in which biotin-conjugated CD19 antibodies are bound by anti-biotin magnetic nanobeads and retained in the separation column, allowing untouched non-B cell populations to be recovered in the flow-through, achieving depletion efficiency up to 99.1% (residual B cell proportion of 0.091%), yielding high-purity, untouched target cell populations suitable for downstream functional and immunological studies.

CD19 is expressed on B lymphocytes from the pro-B stage through plasmablasts (downregulated on mature plasma cells); a pan-B-cell marker.

NoteResearch Use Only (RUO)
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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