Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (27B3), APC (HW717137)

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货号	HW717137
品牌	abinScience
种属反应性	Human
应用	FCM
宿主	Mouse
同种型	IgG2a, lambda
克隆号	27B3
偶联物	APC
克隆类型	Monoclonal
经测试应用	FCM
靶标	CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
内毒素水平	Please contact with the lab for this information.
相关产品	Mouse IgG2a Recombinant Isotype Control Antibody (C1.18.4), APC
Accession号	P32970
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864 3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Flow cytometry

Flow-cytometry using APC anti-human CD70 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD70 monoclonal antibody (Catalog HW717137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (27B3), APC.pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (起始浓度)

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C₂ (终浓度)

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Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (27B3), APC (HW717137)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (27B3), APC (HW717137)

概述

图片

参考文献

产品说明书

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