Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Store at 4°C for 12 months. Protect from light. Do not freeze.
背景
Leukocyte surface antigen CD47 (MER6) is a ~33 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical).
1. Bauer, EM. et al. (2010) Cardiovascular research 88, 471-81. PMID: 20610415
2. Kaur, S. et al. (2013) Scientific reports 3, 1673. PMID: 23591719
3. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621
4. Manna, PP. et al. (2005) The Journal of biological chemistry 280, 29637-44. PMID: 15917238
6. Isenberg, JS. et al. (2008) Annals of surgery 247, 180-90. PMID: 18156939
7. Oldenborg, PA. et al. (2000) Science (New York, N.Y.) 288, 2051-4. PMID: 10856220
Note
For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Flow CytoMetry
Flow-cytometry using APC anti-Mouse CD47 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-Mouse CD47 monoclonal antibody (Catalog: MC359137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
