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Anti-Human IL-21 Recombinant Antibody (SAA0390), APC (HV658137)

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规格:
  • 50T
  • 100T
数量:
  • 概述

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  • 参考文献

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概述
货号HV658137
品牌abinScience
种属反应性Human
应用ELISA, FCM
宿主Mouse
同种型IgG1, kappa
克隆号SAA0390
偶联物APC
克隆类型Monoclonal
经测试应用FCM
靶标IL-21, Interleukin-21, IL21, Za11
内毒素水平Please contact with the lab for this information.
相关产品 Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Accession号Q9HBE4
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Store at 4°C for 12 months. Protect from light. Do not freeze.
背景

Interleukin-21 (IL21) is a ~18 kDa protein. Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection.

1. Parrish-Novak, J. et al. (2000) Nature 408, 57-63. PMID: 11081504
2. Strengell, M. et al. (2004) Journal of leukocyte biology 76, 416-22. PMID: 15178704
NoteFor flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
图片
  • Flow CytoMetry

    Flow-cytometry using APC anti-human IL21 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human IL21 monoclonal antibody (Catalog: HV658137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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