Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), PE (HB209327)

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100T

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货号	HB209327
品牌	abinScience
种属反应性	Human
应用	FCM
宿主	Mouse
同种型	IgG1, kappa
克隆号	SAA0034
偶联物	PE
克隆类型	Monoclonal
经测试应用	FCM
靶标	CD73, 5'-nucleotidase, NT5E, NT5, 5'-NT, Ecto-5'-nucleotidase, NTE
内毒素水平	Please contact with the lab for this information.
相关产品	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), PE
Accession号	P21589
状态	Liquid
保存溶液	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. 1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152 2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138 3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347 4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587 5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Flow CytoMetry

Flow-cytometry using PE anti-human CD73 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD73 monoclonal antibody (Catalog: HB209327, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), PE.pdf

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Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), PE (HB209327)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

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实验相关试剂

Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0034), PE (HB209327)

概述

图片

参考文献

产品说明书

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