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Anti-Human MUC-1 Recombinant Antibody (SAA2216) (HB102407)

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概述
货号HB102407
品牌abinScience
种属反应性Human
应用ELISA, FCM
同种型IgG1, kappa
克隆号SAA2216
经测试应用FCM
靶标PEMT, CA 15-3, H23AG, Peanut-reactive urinary mucin, PEM, EMA, Cancer antigen 15-3, MUC-1, Episialin, MUC1-CT, MUC1-beta, Tumor-associated mucin, CD227, Polymorphic epithelial mucin, KL-6, MUC1-alpha, MUC1, PUM, Krebs von den Lungen-6, MUC1-NT, Tumor-associated epithelial membrane antigen, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin
内毒素水平Please contact with the lab for this information.
纯度>95% by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P15941
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

NoteFor research use only.
图片
  • Flow Cytometry

    Flow-cytometry using anti-human CD227 antibody. MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human CD227 antibody. CD227 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD227 antibody monoclonal antibody (Catalog # HB102407 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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