| 背景 | Protein-glutamine gamma-glutamyltransferase 2 (Zampilimab) is a ~77 kDa protein. Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. 1. Kanchan, K. et al. (2013) The Biochemical journal 455, 261-72. PMID: 239416962. Jeong, EM. et al. (2020) International journal of molecular sciences 21. PMID: 319917883. Nemes, Z. et al. (1997) The Journal of biological chemistry 272, 20577-83. PMID: 92523724. Upchurch, HF. et al. (1991) Journal of cellular physiology 149, 375-82. PMID: 16838746. Hernandez-Fernaud, JR. et al. (2017) Nature communications 8, 14206. PMID: 281983607. Melino, G. et al. (1994) Molecular and cellular biology 14, 6584-96. PMID: 7935379 |
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