Pig CD32b Protein (In Vivo Grade, Ultra-Low Endotoxin) (ICH3022)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This is a recombinant pig (Sus scrofa domesticus) CD32b (Fc gamma receptor IIb / FcgRIIb) protein, corresponding to the sequence recorded under accession ADR51168.1, expressed in human embryonic kidney (HEK) 293 cells. Mammalian HEK293 expression supports native-like folding and post-translational modification of the two extracellular immunoglobulin-like domains, making the protein well suited to binding-kinetics and ligand-interaction studies by SPR, BLI and ELISA. As a soluble recombinant ectodomain rather than a monoclonal antibody, it carries no clone, isotype or host-antibody designation. It is produced to ichor.bio's ultra-low-endotoxin, in-vivo-grade standard (<0.5 EU/mg), available in bulk milligram-to-gram quantities, and supplied for research use only (RUO). The low endotoxin burden is valuable for cell-based functional assays, immunization/tolerance work and any application where LPS contamination would confound results. Typical uses include characterising IgG-Fc binding, screening or ranking antibody Fc variants and engineered Fc domains against a porcine inhibitory receptor, and supporting comparative/translational immunology in the pig model. Researchers often locate this reagent by target (CD32b / FcgRIIb) and species rather than by clone. CD32b (FcgRIIb, gene FCGR2B) is the sole inhibitory member of the Fc gamma receptor family and the only Fc gamma receptor that signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) rather than an activating ITAM. It is a low-affinity receptor for the Fc region of IgG, built from two extracellular immunoglobulin-like domains, a transmembrane segment and an ITIM-bearing cytoplasmic tail. When co-clustered with activating Fc gamma receptors or with the B-cell receptor by immune complexes, its ITIM is phosphorylated and recruits SHIP-1, raising the threshold for cell activation, dampening phagocytosis, and limiting antibody production by B cells. CD32b is the predominant Fc receptor on B lymphocytes and is also expressed on myeloid cells, thereby setting the balance of humoral immunity and innate immune homeostasis. Its structure and inhibitory function are broadly conserved across human, pig and mouse, making porcine CD32b relevant to comparative and translational immunology.