Pig CD16a Protein (In Vivo Grade, Ultra-Low Endotoxin) (ICH3023)

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This product is a recombinant pig (Sus scrofa domesticus) CD16a protein, the extracellular region of the low-affinity IgG Fc receptor FcgammaRIIIa, expressed in human embryonic kidney (HEK) 293 cells. Mammalian HEK293 expression supports native-like folding, disulfide bonding, and glycosylation of the two immunoglobulin-like ectodomains, which is important because FcgammaRIIIa-IgG binding is glycan-sensitive. As a recombinant protein rather than an antibody, it carries no clone or isotype designation (UniProt Q28942). It is well suited to biophysical and functional research: the validated applications are surface plasmon resonance (SPR), biolayer interferometry (BLI), and ELISA, all used to measure Fc-receptor engagement, affinity, and kinetics. The ultra-low endotoxin, in-vivo-grade specification (<0.5 EU/mg) minimizes confounding innate-immune activation in sensitive cell-based and functional readouts, and the material is offered at research bulk scale for RUO. Typical uses include species cross-reactivity studies of therapeutic IgGs, ADCC-effector characterization, and comparative Fc-engineering work spanning pig, human, and other species. CD16a (FcgammaRIIIa), encoded by FCGR3A, is a low-affinity activating receptor for the Fc region of IgG. In the pig, as in humans, it is expressed on natural killer (NK) cells, macrophages (including pulmonary alveolar macrophages), and subsets of monocytes and polymorphonuclear leukocytes. It comprises two extracellular immunoglobulin-like domains, a single transmembrane helix, and associates with the ITAM-bearing FceRIgamma (FCER1G) adapter to transduce activating signals. Engagement by clustered antigen-IgG immune complexes triggers antibody-dependent cellular cytotoxicity (ADCC), degranulation, and cytokine production, while the low monomeric affinity prevents inappropriate activation by free IgG. Binding is enhanced by afucosylated IgG Fc glycoforms. Because pig immunology is central to xenotransplantation, veterinary vaccine, and biomedical model research, pig CD16a is a key tool for characterizing Fc-effector engagement across species.