Mouse CD64 Protein - Biotin (ICH3025B)

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

This is a recombinant Mouse CD64 (FcgammaRI) protein produced in human embryonic kidney (HEK) 293 cells, which supports mammalian folding and glycosylation for native-like conformation and binding activity. It is manufactured to an ultra-low endotoxin specification (<0.5 EU/mg) and supplied bulk (mg-g) as a research-use-only reagent, making it well suited to functional and quantitative binding studies where endotoxin contamination would confound cell-based or in-vivo-adjacent readouts. The protein is validated for surface plasmon resonance (SPR), bio-layer interferometry (BLI), and ELISA, so it functions primarily as a defined binding partner and analyte/ligand rather than as a dosing biologic. Typical uses include measuring Fc-gamma receptor engagement by candidate antibodies and Fc-engineered variants, ranking IgG subclass and Fc-mutant affinities, characterising immune-complex formation, and serving as a capture or detection reagent in receptor-binding assays. Its recombinant, low-endotoxin, bulk format supports reproducible affinity and kinetic characterisation across screening campaigns and lot-to-lot comparisons. All content here is guidance for ichorbio to review before publishing. CD64, also known as Fc-gamma receptor I (FcgammaRI) and encoded by Fcgr1, is the sole high-affinity receptor for monomeric IgG. Unlike the low-affinity FcgammaRII and FcgammaRIII family members, CD64 binds monomeric IgG with nanomolar affinity through its three extracellular immunoglobulin-like domains, with the membrane-distal third domain conferring high-affinity capture. It is expressed constitutively on monocytes, macrophages, and dendritic cells, and is upregulated on neutrophils by interferon-gamma and other inflammatory cues. CD64 signals via the associated FcR gamma-chain, which bears immunoreceptor tyrosine-based activation motifs (ITAMs), triggering phagocytosis, antibody-dependent cellular cytotoxicity, respiratory burst, and cytokine release. In mouse, CD64 is a key effector receptor linking IgG-opsonised targets and immune complexes to myeloid activation, and it contributes to the in-vivo activity of many therapeutic and depleting antibodies.