



| 货号 | HY457013 | ||||
|---|---|---|---|---|---|
| 品牌 | abinScience | ||||
| 种属反应性 | Human | ||||
| 应用 | ELISA, FCM, IHC, IP, Neutralization | ||||
| 同种型 | IgG1, kappa | ||||
| 克隆号 | 8D4-8 | ||||
| 经测试应用 | FCM | ||||
| 靶标 | Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4 | ||||
| 内毒素水平 | Please contact with the lab for this information. | ||||
| 纯度 | >95% as determined by SDS-PAGE. | ||||
| 纯化方式 | Protein A/G purified from cell culture supernatant. | ||||
| Accession号 | P05112 | ||||
| 状态 | Liquid | ||||
| 保存溶液 | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 背景 | Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. 1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171 2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727 3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231 4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247 5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337 6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101 7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895 | ||||
| Note | For research use only. | ||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |

Flow-cytometry using anti-human IL4 antibody.IL4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL4 antibody monoclonal antibody (Catalog # HY457013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human IL4 Antibody (8D4-8).





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