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Anti-Human IL-4 Recombinant Antibody (8D4-8) (HY457013)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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概述
货号HY457013
品牌abinScience
种属反应性Human
应用ELISA, FCM, IHC, IP, Neutralization
同种型IgG1, kappa
克隆号8D4-8
经测试应用FCM
靶标Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P05112
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171
2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727
3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231
4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247
5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337
6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101
7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895
NoteFor research use only.
Isotype ControlMouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
图片
  • Flow Cytometry

    Flow-cytometry using anti-human IL4 antibody.IL4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL4 antibody monoclonal antibody (Catalog # HY457013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human IL4 Antibody (8D4-8).

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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