InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001) (HF827010)

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100ug
1mg

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产品说明书

概述

货号	HF827010
品牌	abinScience
描述	InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001) [Iv0001] (HF827010) is a human monoclonal antibody detecting Interferon alpha-2 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
种属反应性	Human
应用	ELISA, FCM, WB, Neutralization
同种型	IgG1
克隆号	Iv0001
靶标	Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2, IFN-Alpha, IFNA2A, IFNA2B, IFNA2C, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1, IFNA13, IFN-α4, IFN-α5, IFN-α6, IFN-α7, IFN-α8, IFN-αl0, IFN-α14, IFN-α16, IFN-α17, IFN-α21
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
相关产品	pH 7.4 Dilution Buffer
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P01563
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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Bioactivity

Detects Human IFN α2/IFNA2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IFN α2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IFN α2 antibody.SHSY-5Y cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001).
Western blot

Western blot analysis was performed using anti-IFNA2/IFN-alpha-2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 50ng
Lane 2: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 30ng
Lane 3: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 20ng
Lane 4: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 10ng

参考文献

产品说明书

InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

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InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001) (HF827010)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

InVivoMAb Anti-Human IFN-alpha 2/IFNA2 Antibody (Iv0001) (HF827010)

概述

图片

参考文献

产品说明书

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