InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181) (HV514010)

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100ug
1mg

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概述
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概述

货号	HV514010
品牌	abinScience
描述	InVivoMAb Anti-Human RSPO3 Antibody (Iv0181) [Iv0181] (HV514010) is a human monoclonal antibody detecting RSPO3 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
种属反应性	Human
应用	ELISA, FCM, Neutralization
同种型	IgG1, kappa
克隆号	Iv0181
靶标	RSPO3, hPWTSR, hRspo3, PWTSR, R-spondin-3, Thrombospondin type-1 domain-containing protein 2, Protein with TSP type-1 repeat, Roof plate-specific spondin-3, THSD2
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
相关产品	pH 7.4 Dilution Buffer
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q9BXY4
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	R-spondin-3 (RSPO3) is a ~30 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. 1. de Lau, W. et al. (2011) Nature 476, 293-7. PMID: 21727895 2. Glinka, A. et al. (2011) EMBO reports 12, 1055-61. PMID: 21909076 3. Gong, X. et al. (2012) PloS one 7, e37137. PMID: 22615920 4. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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Flow Cytometry

Flow-cytometry using anti-human RSPO3 antibody.RSPO3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human RSPO3 antibody monoclonal antibody (Catalog # HV514010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human RSPO3 Antibody (Iv0181).

参考文献

产品说明书

InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181) (HV514010)

概述

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参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181) (HV514010)

概述

图片

参考文献

产品说明书

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