Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

价格:

规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

JN963033

abinScience

种属反应性

Clostridium perfringens, Streptococcus dysgalactiae

ELISA, Neutralization, WB

IgG2a

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Streptolysin O, SLO, Thiol-activated cytolysin, slo, Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P0C2E9, Q54114

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1) (abinScience Cat# JN963033, RRID:AB_3729019)
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Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

For research use only.

Bioactivity

Detects Clostridium perfringens pfo in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1).
Western blot

Western blot analysis was performed using anti-Theta-toxin monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012)
Lane 2: negative control

产品说明书

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

概述

图片

参考文献

产品说明书

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