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Anti-Staphylococcus aureus Alpha-toxin/hly/AT Recombinant Antibody (2A3.1) (JN998043)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号JN998043
品牌abinScience
种属反应性Staphylococcus aureus
应用ELISA, Neutralization, SPR
同种型IgG2a
克隆号2A3.1
靶标hly, hla, Alpha-hemolysin, Alpha-toxin, Alpha-HL, AT, Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P09616, Q2G1X0
RRID

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1) (abinScience Cat# JN998043, RRID:AB_3729028)
_x005F_x000D_

状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866
2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1)

  • Western blot

    Western blot analysis was performed using anti-a1AT/Serpin A1/SERPINA1 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human a1AT/Serpin A1/SERPINA1 (Catalog No: HF638012)
    Lane 2: negative control

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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