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Anti-Human L-Selectin/CD62L Recombinant Antibody (Dreg-55) (HB644107)

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概述
货号HB644107
品牌abinScience
种属反应性Human
应用FCM, Neutralization
同种型IgG4
克隆号Dreg-55
经测试应用FCM
靶标CD62 antigen-like family member L, Leukocyte adhesion molecule 1, CD62L, LYAM1, TQ1, SELL, Leukocyte-endothelial cell adhesion molecule 1, L-selectin, LAM-1, gp90-MEL, LNHR, LECAM1, Lymph node homing receptor, Leukocyte surface antigen Leu-8
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P14151
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

L-selectin (CD62L/SELL) is a ~42 kDa protein. Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.

1. Bernimoulin, MP. et al. (2003) The Journal of biological chemistry 278, 37-47. PMID: 12403782
2. Mehta-D'souza, P. et al. (2017) The Journal of biological chemistry 292, 2510-2518. PMID: 28011641
3. Wedepohl, S. et al. (2017) Chembiochem : a European journal of chemical biology 18, 1338-1345. PMID: 28489325
NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human CD62L/SELL Antibody (Dreg-55).

  • Flow Cytometry

    Flow-cytometry using anti-human SELL antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human SELL antibody monoclonal antibody (Catalog # HB644107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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