Anti-Human alpha-Fetoprotein/AFP Reference Antibody (Tacatuzumab, RUO) (HY276016)

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概述

货号	HY276016
品牌	abinScience
描述	Tacatuzumab (HY276016) is a research-grade recombinant antibody targeting Alpha-fetoprotein. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG1-kappa
表达系统	Mammalian cells
靶标	Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	P02771
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	Alpha-fetoprotein (AFP) is a ~68 kDa protein. Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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SEC-HPLC

SEC-HPLC detection for Research Grade Tacatuzumab.
Bioactivity

Detects AFP/Alpha-fetoprotein in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Tacatuzumab.
Flow CytoMetry

Flow-cytometry using APC anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011)
Lane 2: negative control

参考文献

产品说明书

Anti-Human alpha-Fetoprotein/AFP Reference Antibody (Tacatuzumab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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质量

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体积

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C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

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Anti-Human alpha-Fetoprotein/AFP Reference Antibody (Tacatuzumab, RUO) (HY276016)

概述

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参考文献

产品说明书

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重组蛋白

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实验相关试剂

Anti-Human alpha-Fetoprotein/AFP Reference Antibody (Tacatuzumab, RUO) (HY276016)

概述

图片

参考文献

产品说明书

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