Protein A/G purified from cell culture supernatant.
Accession号
Q9NZQ7
状态
Liquid
保存溶液
0.01M PBS pH 7.4.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
MPDL3280A, RG7446, 1380723-44-3
背景
Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~33 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi).
_x005F_x000D_
_x005F_x000D_
1. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443
_x005F_x000D_
_x005F_x000D_
2. Mezzadra, R. et al. (2017) Nature 549, 106-110. PMID: 28813410
4. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419
_x005F_x000D_
_x005F_x000D_
5. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298
_x005F_x000D_
_x005F_x000D_
6. Dong, H. et al. (1999) Nature medicine 5, 1365-9. PMID: 10581077
_x005F_x000D_
_x005F_x000D_
7. Hou, J. et al. (2020) Nature cell biology 22, 1264-1275. PMID: 32929201
_x005F_x000D_
Note
For research use only. Not suitable for clinical or therapeutic use.
图片
SEC-HPLC
SEC-HPLC detection for Research Grade Atezolizumab.
Bioactivity
Detects CD274/PD-L1/B7-H1 in indirect ELISAs.
SDS-PAGE
SDS-PAGE for Research Grade Atezolizumab
Flow Cytometry
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog HV974016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
