Anti-Human PD-L1 Reference Antibody (Durvalumab, RUO) (HV974026)

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概述

货号	HV974026
品牌	abinScience
描述	Durvalumab (HV974026) is a research-grade recombinant antibody targeting CD1. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns. Resources ●Flow Cytometry Protocol for PD-L1 (CD274) Detection — Validated protocol on protocols.io
种属反应性	Human
应用	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型	IgG1-kappa
表达系统	Mammalian cells
经测试应用	WB, FCM
靶标	B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274
内毒素水平	< 10 EU/mg
纯度	>95% purity as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q9NZQ7
状态	Liquid
保存溶液	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	MEDI4736, 1428935-60-7
背景	Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~33 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Mezzadra, R. et al. (2017) Nature 549, 106-110. PMID: 28813410 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Burr, ML. et al. (2017) Nature 549, 101-105. PMID: 28813417 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 5. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 6. Dong, H. et al. (1999) Nature medicine 5, 1365-9. PMID: 10581077 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 7. Hou, J. et al. (2020) Nature cell biology 22, 1264-1275. PMID: 32929201 _x005F_x005F_x000D_
Note	For research use only. Not suitable for clinical or therapeutic use.

图片

SEC-HPLC

SEC-HPLC detection for Research Grade Durvalumab.
Bioactivity

Detects Human CD274/PD-L1/B7-H1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog HV974036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Durvalumab
Flow CytoMetry

Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD274 monoclonal antibody (Catalog: HV974026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD274 monoclonal antibody (Catalog: HV974026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD5 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD5 (Catalog No: HY046011), 50ng
Lane 2: recombinant human CD5 (Catalog No: HY046011), 30ng
Lane 3: recombinant human CD5 (Catalog No: HY046011), 20ng
Lane 4: recombinant human CD5 (Catalog No: HY046011), 10ng

参考文献

产品说明书

Anti-Human PD-L1 Reference Antibody (Durvalumab, RUO).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

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实验相关试剂

Anti-Human PD-L1 Reference Antibody (Durvalumab, RUO) (HV974026)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human PD-L1 Reference Antibody (Durvalumab, RUO) (HV974026)

概述

图片

参考文献

产品说明书

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