Anti-SARS-CoV-2 RBD Reference Antibody (Ensovibep, RUO) (VK565336) is a monoclonal antibody detecting SARS-CoV-2 RBD.
种属反应性
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
应用
ELISA, Functional assay, Research in vivo
克隆类型
Monoclonal
同种型
DARpin
生物活性
BA 1 (0.1 uM) significantly increases FAK tyrosine phosphorylation in BRS-3 transfected NCI-H1299 cells [1].
性质
Recombinant
靶标
Spike glycoprotein, Spike, S glycoprotein, RBD
内毒素水平
< 10 EU/mg as determined by LAL method.
纯度
>95% as determined by SDS-PAGE.
纯化方式
Purified by Nickel column.
Accession号
P0DTC2
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.
1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651
7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715
8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763
9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294
10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293
文献
1. Terry W Moody, et al. ML-18 is a non-peptide bombesin receptor subtype-3 antagonist which inhibits lung cancer growth. Peptides. 2015 Feb;64:55-61. [VK565336]
Note
For research use only. Not suitable for clinical or therapeutic use.
