









| 货号 | HP543026 |
|---|---|
| 品牌 | abinScience |
| 描述 |
Anti-Human VSIR/VISTA Antibody (CI-8993) (HP543026) is a research-grade recombinant antibody targeting V-set domain-containing immunoregulatory receptor. Produced in mammalian cells with native-like glycosylation.
Highlights
|
| 种属反应性 | Human |
| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 同种型 | IgG1, kappa |
| 克隆号 | CI-8993 |
| 表达系统 | Mammalian cells |
| 经测试应用 | FCM |
| 靶标 | V-set domain-containing immunoregulatory receptor, C10orf54, VSIR, VISTA, SISP1, Stress-induced secreted protein-1, Platelet receptor Gi24, V-set immunoregulatory receptor, Sisp-1, V-type immunoglobulin domain-containing suppressor of T-cell activation |
| 内毒素水平 | < 10 EU/mg |
| 纯度 | >95% purity as determined by SDS-PAGE. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| Accession号 | Q9H7M9 |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 别名 | VSTB112 |
| 背景 | V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR/VISTA) is a ~33 kDa protein. Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation. 1. Lines, JL. et al. (2014) Cancer research 74, 1924-32. PMID: 24691993 2. Sakr, MA. et al. (2010) Cancer science 101, 2368-74. PMID: 20666777 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |

Detects Human VSIR/VISTA in indirect ELISAs.

SDS-PAGE for Research Grade Anti-Human VSIR/VISTA Antibody (CI-8993).

Flow-cytometry APC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human VSIR monoclonal antibody (Catalog HP543026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry PE using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human VSIR monoclonal antibody (Catalog HP543026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry FITC using anti-human VSIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human VSIR monoclonal antibody (Catalog HP543026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.











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