Anti-SARS-CoV-2 RBD (ADI-62113) (VK565376) is a research-grade recombinant antibody targeting Spike glycoprotein. Produced in mammalian cells with native-like glycosylation.
Highlights
●Research Grade — For PK/PD studies, assay development, and ADA research.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
应用
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主
Human
同种型
IgG1
克隆号
ADI-62113
表达系统
Mammalian cells
克隆类型
Monoclonal
靶标
Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S, 2
内毒素水平
< 10 EU/mg
纯度
>95% purity as determined by SDS-PAGE.
纯化方式
Protein A/G purified from cell culture supernatant.
Accession号
P0DTC2
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.
1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651
