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Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO) (HF879036)

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  • 100ug
  • 1mg
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概述
货号HF879036
品牌abinScience
描述
Infliximab (HF879036) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation.
Highlights
  • Research Grade — For PK/PD studies, assay development, and ADA research.
  • Native Glycosylation — Mammalian expression ensures native-like patterns.
种属反应性Human
应用ELISA, Bioactivity: FACS, Functional assay, Research in vivo
同种型IgG1, kappa
表达系统Mammalian cells
经测试应用FCM
靶标TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
内毒素水平< 10 EU/mg
纯度>95% purity as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号CAS: 170277-31-3
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名TA-650, cA2, 170277-31-3
背景Onercept is a recombinant soluble human tumor necrosis factor-alpha p55 receptor for use in Crohn's disease research.
NoteFor research use only. Not suitable for clinical or therapeutic use.
Isotype ControlHuman IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
图片
  • SEC-HPLC

    SEC-HPLC detection for Research Grade Infliximab.

  • Bioactivity

    Detects Human TNFa/TNF-alpha in indirect ELISAs.

  • SDS-PAGE

    SDS-PAGE for Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

  • Flow CytoMetry

    Flow-cytometry PE using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human TNFa monoclonal antibody (Catalog: HF879036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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