ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB
同种型
IgG1-kappa
表达系统
Mammalian cells
经测试应用
WB
靶标
SKR1, Activin receptor type-1, Activin receptor-like kinase 2, TSR-I, ACVRLK2, ACTR-I, ALK-2, Serine/threonine-protein kinase receptor R1, TGF-B superfamily receptor type I, ACVR1, Activin receptor type I
内毒素水平
< 10 EU/mg
纯度
>95% purity as determined by SDS-PAGE.
纯化方式
Protein A/G purified from cell culture supernatant.
Accession号
Q04771
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
2566473-71-8
背景
Activin receptor type-1 (ACVR1/ALK-2) is a ~57 kDa protein. Bone morphogenetic protein (BMP) type I receptor that is involved in a wide variety of biological processes, including bone, heart, cartilage, nervous, and reproductive system development and regulation. As a type I receptor, forms heterotetrameric receptor complexes with the type II receptors AMHR2, ACVR2A or ACVR2B. Upon binding of ligands such as BMP7 or GDF2/BMP9 to the heteromeric complexes, type II receptors transphosphorylate ACVR1 intracellular domain. In turn, ACVR1 kinase domain is activated and subsequently phosphorylates SMAD1/5/8 proteins that transduce the signal. In addition to its role in mediating BMP pathway-specific signaling, suppresses TGFbeta/activin pathway signaling by interfering with the binding of activin to its type II receptor.
1. Luo, J. et al. (2010) The Journal of biological chemistry 285, 29588-98. PMID: 20628059
2. Chaikuad, A. et al. (2012) The Journal of biological chemistry 287, 36990-8. PMID: 22977237
3. Renlund, N. et al. (2007) The Journal of endocrinology 195, 95-103. PMID: 17911401
4. Fujimoto, M. et al. (2015) Molecular endocrinology (Baltimore, Md.) 29, 140-52. PMID: 25354296
5. Macías-Silva, M. et al. (1998) The Journal of biological chemistry 273, 25628-36. PMID: 9748228
Note
For research use only. Not suitable for clinical or therapeutic use.
Western blot analysis was performed using anti-ACVR1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human ACVR1 (Catalog No: HC323021) Lane 2: Negative control
