Protein A/G purified from cell culture supernatant.
Accession号
P18177
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.
1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489
2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149
3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482
4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933
5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463
6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936
7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356
9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706
Note
For research use only. Not suitable for clinical or therapeutic use.
