ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB
宿主
Human
同种型
IgG1-kappa, fused with humanized vascular endothelial growth factor receptor 1/VEGFR-1 extracellular domain fragment
表达系统
Mammalian cells
克隆类型
Monoclonal
经测试应用
WB
靶标
B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274, L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor
内毒素水平
< 10 EU/mg
纯度
>95% purity as determined by SDS-PAGE.
纯化方式
Protein A/G purified from cell culture supernatant.
Accession号
Q9NZQ7 & P15692
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
2637466-37-4
背景
Sotiburafusp alfa is a bispecific fusion protein, which is a humanized VEGFR-1 extracellular domain fragment (129-228, 1-100 in the current sequence) fused via the peptide linker 101GGSGGSGGSGGSGGS115 to the N-terminus of the heavy chain (116-564) of a humanized IgG1-kappa anti-human PD-L1 heavy chain variant L352>A, L353>A. Sotiburafusp alfa is also an angiogenesis inhibitor.
Note
For research use only. Not suitable for clinical or therapeutic use.
图片
SDS-PAGE
SDS-PAGE for Research Grade Sotiburafusp Alfa.
Bioactivity
Detects recombinant PDL1 (Catalog No: HV974011) in indirect ELISA.
Western blot
Western blot analysis was performed using anti-PDL1 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human PDL1 (Catalog No: HV974011) Lane 2: Negative control
