| 货号 | VK565566 |
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| 品牌 | abinScience |
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| 种属反应性 | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) |
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| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| 宿主 | Human |
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| 同种型 | IgG1-kappa |
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| 克隆号 | VIR-7229 |
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| 表达系统 | Mammalian cells |
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| 经测试应用 | WB |
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| 靶标 | Receptor-Binding Domain, RBD, Spike glycoprotein, S glycoprotein, Peplomer protein, Spike protein S1, S |
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| 内毒素水平 | < 10 EU/mg |
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| 纯度 | >95% purity as determined by SDS-PAGE. |
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| 纯化方式 | Protein A/G purified from cell culture supernatant. |
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| Accession号 | P0DTC2 |
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| 状态 | Liquid |
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| 保存溶液 |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| 别名 | VIR-7229, VIR7229, VIR7229 |
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| 背景 | Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors. 3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 336070864. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 345618875. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 320758776. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 322213068. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 367797639. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 3308229410. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293 |
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| 文献 | 1. Rosen LE, et al. A potent pan-sarbecovirus neutralizing antibody resilient to epitope diversification. Cell. 2024 Dec 12;187(25):7196-7213.e26. [VK565566] |
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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