Protein A/G purified from cell culture supernatant.
Accession号
P07333
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
RG7155, RO5509554, 1448221-67-7
背景
Macrophage colony-stimulating factor 1 receptor (CD115/CSF1R) is a ~107 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion.
1. Wei, S. et al. (2010) Journal of leukocyte biology 88, 495-505. PMID: 20504948
2. Guo, L. et al. (2019) American journal of human genetics 104, 925-935. PMID: 30982609
3. Oosterhof, N. et al. (2019) American journal of human genetics 104, 936-947. PMID: 30982608
Note
For research use only. Not suitable for clinical or therapeutic use.
Flow-cytometry using PE anti-human CD115 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD115 monoclonal antibody (Catalog: HY035026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
