Human CTLA-4 Recombinant Protein (C-His) (HB651011)
概述
图片
参考文献
产品说明书
概述
货号
HB651011
品牌
abinScience
描述
Recombinant Human CD152/CTLA4 Protein, C-His (HB651011) expressed in Mammalian Cells, spanning Met1-Asp161. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P16410
蛋白长度
Met1-Asp161
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
经测试应用
WB
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
18.55 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Cytotoxic T-lymphocyte protein 4 (CD152/CTLA4) is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. CD152 is the therapeutic target of ipilimumab (Yervoy).
2. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502
3. Teft, WA. et al. (2006) Annual review of immunology 24, 65-97. PMID: 16551244
4. Linsley, PS. et al. (1991) The Journal of experimental medicine 174, 561-9. PMID: 1714933
5. Zheng, Y. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 1683-91. PMID: 18641304
6. Ramagopal, UA. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E4223-E4232. PMID: 28484017
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human CD152/CTLA4 Protein, C-His
Bioactivity
Detects Human CD152/CTLA4 Antibody in indirect ELISAs.
Bioactivity
Detects Ipilimumab in indirect ELISAs.
Bioactivity
Detects Nurulimab in indirect ELISAs.
Bioactivity
Detects Cadonilimab in indirect ELISAs.
Bioactivity
Detects Botensilimab in indirect ELISAs.
Bioactivity
Detected by Research Grade Gotistobart (Cat No.: HB651266) in indirect ELISA.
Western blot
Detected by Anti-CD152/CTLA4 monoclonal antibody (Catalog No: HS870396)
Bioactivity
Detected by Research Grade Quavonlimab (Catalog No: HB651066) in indirect ELISA
Bioactivity
Detected by Anti-Human CD152/CTLA4 Antibody (HL32) (Catalog No: HB651407) in indirect ELISA
Bioactivity
Detected by SAA1104 (Catalog No: HB651013) in indirect ELISA.
Bioactivity
Detected by Pavunalimab (Catalog No: HB613086) in indirect ELISA.
Bioactivity
Detected by Lorigerlimab (Catalog No: HB651086) in indirect ELISA.
Bioactivity
Detected by Tuvonralimab (Catalog No: HB651096) in indirect ELISA.
Bioactivity
Detected by Porustobart (Catalog No: HB651246) in indirect ELISA.
Bioactivity
Detected by Firastotug (Catalog No: HB651256) in indirect ELISA.
Bioactivity
Detected by Muzastotug (Catalog No: HB651276) in indirect ELISA.
Bioactivity
Detected by Sovipostobart (Catalog No: HB651286) in indirect ELISA.
Bioactivity
Detected by Evalstotug (Catalog No: HB651296) in indirect ELISA.
Bioactivity
Detected by Vilastobart (Catalog No: HB651306) in indirect ELISA.
Bioactivity
Detected by Tazlestobart (Catalog No: HB651316) in indirect ELISA.
Bioactivity
Immobilized HW630021 Human CD80/B7-1 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB651011 Human CD152/CTLA4 Recombinant Protein (C-His), the EC 50 is 250 ng/mL.
Bioactivity
Immobilized MW630041 Mouse CD80 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB651011 Human CD152/CTLA4 Recombinant Protein (C-His), the EC 50 is 354 ng/mL.
