Human CD27 Ligand/TNFSF7/CD70 Recombinant Protein (C-Fc) (HB796011)
概述
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参考文献
产品说明书
概述
货号
HB796011
品牌
abinScience
描述
Recombinant Human CD27 Protein, C-Fc (HB796011) expressed in Mammalian Cells, spanning Met1-Ile192.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P26842
蛋白长度
Met1-Ile192
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
经测试应用
WB
内毒素水平
Please contact with the lab for this information.
纯度
>95% as determined by SDS-PAGE.
预测分子量
47.84 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue.
1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
Note
For research use only.
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SDS-PAGE
SDS-PAGE for Recombinant Human CD27 Protein, C-Fc
Bioactivity
Detects Varlilumab in indirect ELISAs.
Bioactivity
Detects Human CD27 Antibody (5F24) in indirect ELISAs.
Western blot
Detected by Anti-CD27 monoclonal antibody (Catalog No: HP628207)
Bioactivity
Immobilized HB796011 Human CD27 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HW717011 Human CD70 Recombinant Protein (N-Fc), the EC 50 is 5.78 μg/mL.
Bioactivity
Immobilized MW717021 Mouse CD70 Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HB796011 Human CD27 Recombinant Protein (C-Fc), the EC 50 is 310 ng/mL.
