Human CD3 epsilon Recombinant Protein (C-Fc) (HY057031)
概述
图片
参考文献
产品说明书
概述
货号
HY057031
品牌
abinScience
描述
Recombinant Human CD3E Protein, C-Fc (HY057031) expressed in Mammalian Cells, spanning Met1-Asp126. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
P07766
蛋白长度
Met1-Asp126
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
40.56 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).
1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938
2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002
3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098
4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325
5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human CD3E Protein, C-Fc
Bioactivity
Detected by SAA2242 (Catalog No: HY057033) in indirect ELISA.
Bioactivity
Detected by SAA2242 (Catalog No: HY057043) in indirect ELISA.
Bioactivity
Detected by SAA2242 (Catalog No: HY057033) in indirect ELISA.
Bioactivity
Detected by SAA2242 (Catalog No: HY057043) in indirect ELISA.
Bioactivity
Detected by Hpn536 (Catalog No: HC496106) in indirect ELISA.
