Recombinant Human CD151 Protein, N-Fc (PX-P6192) expressed in Mammalian Cells, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
Mammalian Cells Expression — Optimized for functional studies.
表达系统
Mammalian Cells
Accession号
P48509
蛋白长度
Ala113-Arg221
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
40.3 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CD151 antigen is a ~28 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis. Via interaction with JAM-A/F11R and integrin ITGA3:ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process. Regulates the glycosylation pattern of ITGA3:ITGB1 thereby modulating its activity.
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2. Rocha-Perugini, V. et al. (2014) European journal of immunology 44, 1967-75. PMID: 24723389
3. Te Molder, L. et al. (2019) Journal of cell science 132. PMID: 31488507
4. Hong, IK. et al. (2012) The Journal of biological chemistry 287, 32027-39. PMID: 22843693
5. Thölmann, S. et al. (2022) Cellular and molecular life sciences : CMLS 79, 88. PMID: 35067832
6. Baldwin, G. et al. (2008) The Journal of biological chemistry 283, 35445-54. PMID: 18852263
7. Karamatic Crew, V. et al. (2004) Blood 104, 2217-23. PMID: 15265795
