Recombinant Human IL12A/IL-12 p35/NKSF1 Protein, C-Strep (PX-P5777)
概述
图片
参考文献
概述
货号
PX-P5777
品牌
ProteoGenix
描述
Recombinant Human IL12A/IL-12 p35/NKSF1 Protein, C-Strep (PX-P5777) expressed in Mammalian Cells, Purity: >90% as determined by SDS-PAGE..
Highlights
High Purity (>90% as determined by SDS-PAGE.) — Verified by SDS-PAGE for downstream accuracy.
Mammalian Cells Expression — Optimized for functional studies.
表达系统
Mammalian Cells
Accession号
P29459
蛋白长度
Arg23-Ser219
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
27.83 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interleukin-12 subunit alpha (IL12A/IL-12 p35) is a ~24 kDa protein. Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes.
1. Heufler, C. et al. (1996) European journal of immunology 26, 659-68. PMID: 8605935
2. Presky, DH. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 14002-7. PMID: 8943050
3. Wolf, SF. et al. (1991) Journal of immunology (Baltimore, Md. : 1950) 146, 3074-81. PMID: 1673147
4. Gubler, U. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 4143-7. PMID: 1674604
5. Bacon, CM. et al. (1995) The Journal of experimental medicine 181, 399-404. PMID: 7528775
6. Bacon, CM. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7307-11. PMID: 7638186
