ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
31.24 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Protein sna, SNAI1, Zinc finger protein SNAI1, Protein snail homolog 1, SNAH
背景
Zinc finger protein SNAI1 is a ~29 kDa protein. Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. The N-terminal SNAG domain competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (in vitro). During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription. SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits.
1. Batlle, E. et al. (2000) Nature cell biology 2, 84-9. PMID: 10655587
2. Yook, JI. et al. (2005) The Journal of biological chemistry 280, 11740-8. PMID: 15647282
3. Lin, Y. et al. (2010) The EMBO journal 29, 1803-16. PMID: 20389281
4. Lin, T. et al. (2010) Oncogene 29, 4896-904. PMID: 20562920
5. Zhang, K. et al. (2012) The EMBO journal 31, 29-43. PMID: 21952048
6. Jin, Y. et al. (2015) Cancer letters 362, 70-82. PMID: 25827072
8. Tortorici, M. et al. (2013) ACS chemical biology 8, 1677-82. PMID: 23721412
9. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638
10. Hu, CT. et al. (2010) The FEBS journal 277, 1202-18. PMID: 20121949
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human SNAI1 Protein, N-His
