Human TIGIT Recombinant Protein (C-His) (HS739011)
概述
图片
参考文献
产品说明书
概述
货号
HS739011
品牌
abinScience
描述
Recombinant Human TIGIT Protein, C-His (HS739011) expressed in Mammalian Cells, spanning Met1-Pro141.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
Q495A1
蛋白长度
Met1-Pro141
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>95% as determined by SDS-PAGE.
预测分子量
16.39 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
VSTM3, TIGIT, V-set and transmembrane domain-containing protein 3, VSIG9, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
背景
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a ~26 kDa protein. Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades.
2. Liu, S. et al. (2013) Cell death and differentiation 20, 456-64. PMID: 23154388
3. Li, M. et al. (2014) The Journal of biological chemistry 289, 17647-57. PMID: 24817116
4. Reches, A. et al. (2020) Journal for immunotherapy of cancer 8. PMID: 32503945
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human TIGIT Protein, C-His
Bioactivity
Detects Human TIGIT Antibody in indirect ELISAs.
Bioactivity
Detects Tamgiblimab in indirect ELISAs.
Bioactivity
Detects Etigilimab in indirect ELISAs.
Bioactivity
Detects Vibostolimab in indirect ELISAs.
Bioactivity
Detected by Belrestotug (Catalog No: HS739076) in indirect ELISA.
Bioactivity
Detected by Dargistotug (Catalog No: HS739086) in indirect ELISA.
Bioactivity
Detected by Ralzapastotug (Catalog No: HS739096) in indirect ELISA.
Bioactivity
Detected by Lancastotug (Catalog No: HS739246) in indirect ELISA.
Bioactivity
Detected by Lucorafusp alfa (Catalog No: HS739266) in indirect ELISA.
Bioactivity
Immobilized HS739011 Human TIGIT Recombinant Protein (C-His) at 2 μg/mL(100 μL/well) can bind HB135021 Human CD155/PVR Recombinant Protein (C-Fc), the EC 50 is 10.8 μg/mL.
