ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
23.21 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Calcium uniporter protein, mitochondrial (MCU) is a ~39 kDa protein. Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix. MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells.
1. Baughman, JM. et al. (2011) Nature 476, 341-5. PMID: 21685886
2. De Stefani, D. et al. (2011) Nature 476, 336-40. PMID: 21685888
3. Drago, I. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 12986-91. PMID: 22822213
4. Tarasov, AI. et al. (2012) PloS one 7, e39722. PMID: 22829870
5. Alam, MR. et al. (2012) The Journal of biological chemistry 287, 34445-54. PMID: 22904319
6. Mallilankaraman, K. et al. (2012) Cell 151, 630-44. PMID: 23101630
7. Mallilankaraman, K. et al. (2012) Nature cell biology 14, 1336-43. PMID: 23178883
8. Chaudhuri, D. et al. (2013) eLife 2, e00704. PMID: 23755363
9. Hoffman, NE. et al. (2013) Cell reports 5, 1576-1588. PMID: 24332854
10. Patron, M. et al. (2014) Molecular cell 53, 726-37. PMID: 24560927
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human MCU Protein, N-His.
