Human USP7 Recombinant Protein (N-His) (HV097012)

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1mg

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概述

货号	HV097012
品牌	abinScience
描述	Recombinant Human USP7 Protein, N-His (HV097012) expressed in E. coli, spanning Gly202-Asn1102. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
表达系统	E. coli
Accession号	Q93009
蛋白长度	Gly202-Asn1102
应用	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属	Homo sapiens (Human)
性质	Recombinant
内毒素水平	Please contact with the lab for this information.
纯度	>90% as determined by SDS-PAGE.
预测分子量	107.31 kDa
状态	Lyophilized
保存溶液	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名	Ubiquitin-specific-processing protease 7, Deubiquitinating enzyme 7, Ubiquitin thioesterase 7, USP7, Herpesvirus-associated ubiquitin-specific protease, HAUSP, Ubiquitin carboxyl-terminal hydrolase 7
背景	Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ~128 kDa protein. Hydrolase that deubiquitinates target proteins such as ARMC5, FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. 1. Li, M. et al. (2002) Nature 416, 648-53. PMID: 11923872 2. Li, M. et al. (2004) Molecular cell 13, 879-86. PMID: 15053880 3. van der Horst, A. et al. (2006) Nature cell biology 8, 1064-73. PMID: 16964248 4. Song, MS. et al. (2008) Nature 455, 813-7. PMID: 18716620 5. Zhang, J. et al. (2014) Nature communications 5, 5059. PMID: 25283148 6. Cui, H. et al. (2015) Nature communications 6, 6752. PMID: 25865756 7. Ding, X. et al. (2015) PloS one 10, e0145023. PMID: 26678539 8. Jiang, L. et al. (2017) The Journal of biological chemistry 292, 13296-13311. PMID: 28655758 9. Yan, G. et al. (2021) Journal of cellular and molecular medicine 25, 3149-3159. PMID: 33544460 10. Vega, M. et al. (2022) The Journal of biological chemistry 298, 101750. PMID: 35216969
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human USP7 Protein, N-His

参考文献

产品说明书

Human USP7 Recombinant Protein (N-His).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Human USP7 Recombinant Protein (N-His) (HV097012)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Human USP7 Recombinant Protein (N-His) (HV097012)

概述

图片

参考文献

产品说明书

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