ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Remove endotoxin and sterilization is required for cell assay.
纯度
>90% as determined by SDS-PAGE.
预测分子量
38.93 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Heat shock factor protein 4 (HSF4) is a ~53 kDa protein. Heat-shock transcription factor that specifically binds heat shock promoter elements (HSE). Required for denucleation and organelle rupture and degradation that occur during eye lens terminal differentiation, when fiber cells that compose the lens degrade all membrane-bound organelles in order to provide lens with transparency to allow the passage of light. In this process, may regulate denucleation of lens fiber cells in part by activating DNASE2B transcription. May be involved in DNA repair through the transcriptional regulation of RAD51. May up-regulate p53/TP53 protein in eye lens fiber cells, possibly through protein stabilization.
1. Cui, X. et al. (2012) Biochimica et biophysica acta 1822, 1308-15. PMID: 22587838
2. Cui, X. et al. (2013) Biochimica et biophysica acta 1832, 1167-72. PMID: 23507146
3. Gao, M. et al. (2017) Cell death & disease 8, e3082. PMID: 28981088
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human HSF4 Protein, N-GST
