Human AOC3/VAP-1 Recombinant Protein (C-His) (HS721011)
概述
图片
参考文献
产品说明书
概述
货号
HS721011
品牌
abinScience
描述
Recombinant Human AOC3/VAP-1 Protein, C-His (HS721011) expressed in Insect Cells, spanning Gly27-Asn763. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Insect cells
Accession号
Q16853
蛋白长度
Gly27-Asn763
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
预测分子量
84.75 kDa
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Amine oxidase [copper-containing] 3 (AOC3/VAP-1) is a ~84 kDa protein. Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia. Has a preference for the primary monoamines methylamine and benzylamine. Could also act on 2-phenylethylamine but much less efficiently. At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion.
1. Kaitaniemi, S. et al. (2009) Cellular and molecular life sciences : CMLS 66, 2743-57. PMID: 19588076
2. Bligt-Lindén, E. et al. (2013) Journal of medicinal chemistry 56, 9837-48. PMID: 24304424
3. Smith, DJ. et al. (1998) The Journal of experimental medicine 188, 17-27. PMID: 9653080
4. Salmi, M. et al. (1997) The Journal of experimental medicine 186, 589-600. PMID: 9254657
Note
For research use only.
图片
SDS-PAGE
SDS-PAGE for Recombinant Human AOC3/VAP-1 Protein, C-His
Bioactivity
Detects Human VAP-1/AOC3 Antibody (TK8-14) in indirect ELISAs.
