Human TMPRSS2 Recombinant Protein (N-Fc) (HT176011)
概述
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参考文献
产品说明书
概述
货号
HT176011
品牌
abinScience
描述
Recombinant Human TMPRSS2 Protein, N-Fc (HT176011) expressed in Mammalian Cells, spanning Trp106-Arg255. Purity: >90% by SDS-PAGE.
Highlights
●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
表达系统
Mammalian cells
Accession号
O15393
蛋白长度
Trp106-Arg255
应用
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
种属
Homo sapiens (Human)
性质
Recombinant
内毒素水平
Please contact with the lab for this information.
纯度
>90% as determined by SDS-PAGE.
状态
Lyophilized
保存溶液
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Transmembrane protease serine 2 (TMPRSS2) is a ~53 kDa protein. Plasma membrane-anchored serine protease that cleaves at arginine residues. Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells. In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia.
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1. Bestle, D. et al. (2020) Life science alliance 3. PMID: 32703818
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2. Fraser, BJ. et al. (2022) Nature chemical biology 18, 963-971. PMID: 35676539
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3. Wang, H. et al. (2023) Nature communications 14, 7574. PMID: 37990007
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4. McCallum, M. et al. (2024) Cell 187, 4231-4245.e13. PMID: 38964328
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5. Lucas, JM. et al. (2014) Cancer discovery 4, 1310-25. PMID: 25122198
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6. Wilson, S. et al. (2005) The Biochemical journal 388, 967-72. PMID: 15537383
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7. Ko, CJ. et al. (2015) Cancer research 75, 2949-60. PMID: 26018085
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Note
For research use only.
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SDS-PAGE
SDS-PAGE for Recombinant Human TMPRSS2 Protein, N-Fc
